Journal: Cell reports

Article Title: Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer’s disease

doi: 10.1016/j.celrep.2023.113392

Figure Lengend Snippet:

Article Snippet: rabbit anti-CAPG , Proteintech , Cat# 10194-1-AP; RRID: AB_2069179.

Techniques: Control, Recombinant, Transfection, Isolation, Transgenic Assay, Software

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Distinct VASP tetramers synergize in the processive elongation of individual actin filaments from clustered arrays

doi: 10.1073/pnas.1703145114

Figure Lengend Snippet: VASP does not require actin ATP hydrolysis of terminal subunits for filament elongation. (A) TIRF images of 4 µM ADP-actin (23% Alexa 488 labeled) polymerized in 1× ADP-TIRF buffer in the absence (Top) or presence (Lower) of 200 nM VASP-4M. Time is given in seconds. (B) Elongation rates of ADP-actin in the presence of increasing concentrations of VASP-4M in solution. (C) Comparison of elongation rates of ADP-actin or ATP-actin in absence or presence of 200 nM VASP-4M. (D) TIRFM images of 4 µM ADP-actin (Top) and 1.0 µM ATP-actin (Lower) polymerized in presence of VASP-4M–coated microspheres and 40 nM CP. (E) Comparison of elongation rates from ADP-G-actin and ATP-G-actin in the presence VASP-4M–derivatized beads. Note similar decrease in presence of ADP-actin for spontaneous and VASP-mediated actin assembly. (A and D) Time is given in seconds. (Scale bars, 20 µm.) (B, C, and E) Data correspond to means ± SD. Fifteen actin control filaments and 30 filaments for all other data points in presence of VASP-4M were analyzed.

Article Snippet: The TIRFM assays were initiated by addition of ATP-G-actin (1 µM final concentration, 23% Alexa 488 labeled) or ADP-G-actin (4 µM final concentration, 23% Alexa 488 labeled) and by flushing of the mixtures into mPEG-silane ( M r 2,000) (Laysan Bio)-precoated flow chambers.

Techniques: Labeling